Develop Your Conflict Resolution Skills Essay Example | Topics and Well Written Essays - 750 words

Develop Your Conflict Resolution Skills - Essay Example Injustice: this is the perceived mistreatment by others or the world at large. This attitude leads to the individual to identify something as unfair and thereby engage in retaliatory acts. Vulnerability: it revolves around a person’s perception that he/she is the subject to internal/external dangers where control lacks to supply safety. Distrust: it focuses on presumed hostility and malign intent of others. This is an expectation that others will hurt, cheat, abuse, humiliate or take advantage. Helplessness: this is a conviction that the carefully planned and executed plans/actions will fail to produce desired outcomes. This leads to a person perceiving that he/she cannot accomplish anything. The conflict is solved when the inconsistency between actions and wishes of parties are resolved. Returning to the Sally and Dave conundrum, as the managers duty at the reservations call center is to ensure this conflict is resolved. Therefore, the managers first action after analyzing the conflict and putting all parties into consideration is to handle the issue separately, and find a solution after listening to each party’s complain. First, it is hard to tell who is telling the truth since each proposal is a complete opposite of the other party’s proposal. So, after meeting with Dave, the manager should ask him to be punctual with the time he departs from the center. He cannot stay at the center past 7:30. This would mean that he has to complete his job by seven o’clock thus giving him some time to tidy up the cubicle and turn off his computer. He should keep his stuff and personal effects to a minimized and limited to one side of the work station. Failure to honor this, he should be stripped of these permissions. He should keep his personal belongings on one side of the drawer and not leave them scattered in every corner of the drawer. Above all, responsibility and respect towards Sally would be demanded from Dave. Sally

Protein Quantitation Lab Report Example | Topics and Well Written Essays - 1250 words

Protein Quantitation - Lab Report Example The following is a report on an experiment conducted to determine protein concentration of unknown samples using this method. Bradford Protein Concentration Assay Accurate protein quantitation is paramount to all experiments that are related to proteins in a lot of research topics in molecular biology, developmental biology, cell biology, neuroscience, and biochemistry. Different techniques have been developed to quantitate proteins in the last century, both for the total protein content and a single protein. Total protein content quantitation methods include Bradford assays. Bradford assay, which was initially described by Dr. Marion Bradford in 1976, is one of the commonly used methods to determine protein concentration. This method relies on formation of a complex between proteins in solution and the Coomassie brilliant blue G-250 dye. This dye exists in four different ionic forms. The more anionic blue form binds to proteins and has an absorbance at 590 nm. Protein concentrations can be known by determining the amount of dye in the blue ionic form, and by measuring the absorbance of the solution at 595 nm using a spectrophotometer (Becker, Caldwell & Zachgo, 1996). This dye binds mostly to arginine, tryptophan, tyrosine, histidine, and phenylalanine residues of the protein Materials and Reagents Protein standard: 1mg/mL Albumin Bradford reagent Distilled water Test samples A, B and C (Unknown protein) One 96-well plate Procedure First, the albumin standard solutions were prepared as follows: Concentration Albumin Distilled Water 0% 0ul 100ul 25% 25ul 75ul 50% 50ul 50ul 75% 75ul 25ul 100% 100ul 0ul Then the Bradford reagents were diluted with 300 ul (Bradford): 1500ul (Distill Water) (1:5 ratio). In the first trial, 180ul of the diluted Bradford reagents was added into the 96 well plate. Then 0%, 25%, 50%, 75%, 100% and Sample A, B, C, each 5ul was added to each well hole which contained 180ul of diluted Bradford reagent. These were tested with spectrophotom eter and the results recorded. The experiment was repeated again the same way. In addition, Sample C was diluted with 3 different ratios as follows: 1:9 (Sample C: Distill Water) 1:99 (Sample C: Distill Water) 5:95 (Sample C: Distill Water) The results were recorded after the solutions were tested with a spectrophotometer. A standard curve of absorbance versus concentration protein was drawn. Results The results were recorded as follows: First Trial Results: 0% 25% 50% 75% 100% Sample A Sample B Sample C 0.092 0.145 0.161 0.169 0.162 0.095 0.161 0.763 Second Trial Results: 0% 25% 50% 75% 100% Sample A Sample B Sample C Sample C (1:9) Sample C (1:99) Sample C (5:95) 0.113 0.188 0.218 0.217 0.210 0.116 0.200 0.670 0.244 0.118 0.152 The responses of the standards were used to plot a standard curve. Absorbance values of unknown samples were then interpolated onto the plot for the standard curve to determine their concentrations as shown in the graph below. Discussion From the graph abov e, the optimum measurement wavelength for this assay is on sample C. Thus sample C has the highest protein concentration. Sample A has the same absorbance as 0% albumin and Sample B has the same absorbance as 50% albumin. It can therefore be concluded that sample A and Sample B have 0% and 50% protein concentra