Sunday, May 17, 2020

Case Study of Brady v. Salt Lake City Essay - 610 Words

Significant Facts Two Utah sisters, Maria and Jan Brady posted a message on Facebook in support of anti-globalization and anti-genetically organism (GMO) activists against McDonalds restaurants, who had incited riots in Europe. The sister’s message, sent to over 500 â€Å"friends†, asking them to join the sisters in two days at 10 p.m. at a McDonalds near the sister’s apartment for a â€Å"night of riot, pillage, and fun†. A secondary message was sent out asking the participants to bring items that could be used as weapons or cause property damage. However, one of the â€Å"friends† that the message was sent to turned the postings over to the Salt Lake Police Department. SLCPD officers set up an operation at the scheduled place and time the sister’s†¦show more content†¦Similarly, a State’s statute will found constitutional if they are not arbitrary and a reasonable attempt of the state to protect the public within vested stat e police powers. In Gitlow v. New York, the petitioner was charged with criminal anarchy for advocating a socialist reform in the United States. In 1919, he had published a socialist manifesto and was preparing a mass distribution throughout New York City. The Court held that the State’s statute fell within ordinary scrutiny, and a reasonable means of exercising the police powers of the State. Justice Sanford held that a state has the power to prevent disturbing the peace and restrict speech that has the potential to incite violence, even though there may be no immediate threat of such action. Gitlow set the precedent for the â€Å"Bad Tendency† test, where freedom of speech and press does not give an individual the unmitigated right to any speech or publication without being held responsible for the results. However, in 1969, the Supreme Court held that the clear and present danger test was not an acceptable standard for interpreting the First Amendment. In Brandenbur g v. Ohio, Brandenburg was convicted under the Ohio Criminal Syndicalism Act, which prohibits â€Å"advocating the duty, necessity, or priority of crime, sabotage, violence, or unlawful methods of terrorism as a means of accomplishing industrial or political reform† (Epstein Walker, p. 223). The Court held that although the Act didShow MoreRelatedStephen P. Robbins Timothy A. Judge (2011) Organizational Behaviour 15th Edition New Jersey: Prentice Hall393164 Words   |  1573 Pages—San Diego State University Timothy A. Judge —University of Notre Dame i3iEi35Bj! Boston Columbus Indianapolis New York San Francisco Upper Saddle River Amsterdam Cape Town Dubai London Madrid Milan Munich Paris Montreal Toronto Delhi Mexico City Sao Paulo Sydney Hong Kong Seoul Singapore Taipei Tokyo Editorial Director: Sally Yagan Director of Editorial Services: Ashley Santora Acquisitions Editor: Brian Mickelson Editorial Project Manager: Sarah Holle Editorial Assistant: Ashlee BradburyRead MoreCost Accounting134556 Words   |  539 PagesAccounting, 5/e 1–9. In decision making, managers or supervisors may wish to take actions that are not economically justifiable. In most cases, upon receipt of a well-developed cost analysis, a production manager is satisfied whether an action is feasible. If the action is not economically justifiable, the matter is dropped without conflict. In a few cases, however, managers may wish to pursue a project because of personal reasons, and hope to have an economic analysis to support it. In these situations

Wednesday, May 6, 2020

Literary Techniques Used in Mother to Son by Langston...

â€Å"Mother to Son,† written by Langston Hughes, is a short poem about a mother who is teaching her son about perseverance and determination by using the image of a staircase. She explains that even though life has given her many hardships, she continues forward and she urges her son to do the same. In â€Å"Mother to Son,† Langston Hughes uses an extended metaphor, imagery, dialect, and structure to paint a picture of a weary mother who wants her son to persevere through the hardships of life. The theme of this poem is determination and the value of experience. The mother is telling her son that no matter what happens, he should never give up. Even though this is a rather sad poem, there is a glimpse of hope because she never gave up. She never†¦show more content†¦A staircase that is broken would not be in a wealthy person’s home, so this also shows us that she is poor. In the 1920’s, which was when this poem was written, many African Americans were moving from the South into the cities for a chance at making a living. Most of them were very poor and lived in poverty, which is why the staircases would be broken down, bare, and no carpet. The title, â€Å"Mother to Son,† tells us that this is a mother giving her son advice. Langston Hughes opens the poem by saying, â€Å"Well, son, I’ll tell you:† (1), which could mean that the son had asked her about her life or that maybe he was complaining about his current struggles. The mother then begins to tell her son that her life has not been easy, but she never gives up and she urges her son to do the same: So boy, don’t you turn back. Don’t you set down on the step ‘Cause you finds it’s kinder hard. Don’t you fall now-- The mother is telling her son that he should never turn around or sit down just because it gets tough, and she uses herself as an example. You can see the determination that this mother has. She keeps trying and persevering through her troubles, even when it seems like there is no hope, â€Å"And sometimes goin’ in the dark / Where there ain’t been no light.† (12-13). â€Å"And sometimes goin’ in the dark† (12) could mean two things: she felt hopeless at some points in her life, or that it was hard to see the way. If a place is completely dark with no trace light, it isShow MoreRelatedLangston Hughes : Jazz Poetry And Harlem Renaissance1212 Words   |  5 PagesLangston Hughes Jazz Poetry and Harlem Renaissance Langston Hughes was an African American poet who was born on 01 February 1902 in Joplin, Missouri. His parents separated and later divorced during his childhood. Subsequently he was raised predominantly by his maternal grandmother. His grandparents were politically active and supporters of the abolition of slavery. They were activists in the movement for voting rights for African Americans. Through their active involvement in his upbringingRead MoreLangston Hughes : The Black Writers Of The Harlem Renaissance1488 Words   |  6 Pageswriters of the 1900’S is Langston Hughes. While many writers focused on one style or category of writing, Langston Hughes is the most versatile of all of the writers from the Harlem Renaissance. While the state of Missouri is known for its largest cities such as St. Louis and Kansas City and for famous attractions such as the Gateway Arch, the state is also the birthplace of one of the most well-known writers of the early Twentieth Century. James Mercer Langston Hughes is an American author, writerRead MoreReoccurring Themes in the Work of Langston Hughes Essay1649 Words   |  7 PagesLangston Hughes is an extremely successful and well known black writer who emerged from the Harlem Renaissance (â€Å"Langston Hughes† 792). He is recognized for his poetry and like many other writers from the Harlem Renaissance, lived most of his life outside of Harlem (â€Å"Langston Hughes† 792). His personal experiences and opinions inspire his writing intricately. Unlike other writers of his time, Hughes expresses his discontent with black oppression and focuses on the hardships of his people. Hughes’Read MoreThe Ways Of White Folks By Langston Hughes2313 Words   |  10 Pagesâ€Å"Humor is laughing at what you haven t got when you ought to have it† - Langston Hughes. This quote finds relevance in Langston Hughes 1934 novel â€Å"the Ways of White Folks†, as he adds a numerous amount of blunt and hidden jokes within the novel. These jokes are meant to laugh at the inequality between white people and black people within America. Hughes mocking of the inability of the American population to coexist is exemplified through the theme of the novel that the emotional disparity betweenRead MoreLiterary Analysis Of Gwendolyn Brooks s The Bean Eaters 1907 Words   |  8 PagesWith the publication of her second volume of poetry, Annie Allen (1949), she became the first black American writer to win a Pulitzer Prize. Noted traditional forms and poignant evocation of urban black experience, Brooks emerged as a leading black literary figure during the 1950s and 1960s. her lyrical poetry addresses racial injustice, poverty, and the private struggles of young black women with exceptional precision, psychological depth, and authenticity. In addition to Annie Allen, Brooks is bestRead MoreOrder My Steps2359 Words   |  10 PagesI can say one thing for sure,† Life for me ain’t been no crystal stairs.† (Hughes, 1995) After receiving this assignment, I stepped back and took a good look at my life. I contemplated the different ways I have grown and changed. I began to recall certain parts of my life that may have had an effect on the experiences that I had to endure in life. With all my life experience, I am able to identify all three domains of developmental in my life. As this paper progresses, you will learn about my familyRead MoreNot Without Laughter Study Guide9912 Words   |  40 PagesBookRags Literature Study Guide Not Without Laughter by Langston Hughes For the online version of BookRags Not Without Laughter Literature Study Guide, including complete copyright information, please visit: http://www.bookrags.com/studyguide-not-without-laughter/ Copyright Information  ©2000-2012 BookRags, Inc. ALL RIGHTS RESERVED. The following sections of this BookRags Literature Study Guide is offprint from Gales For Students Series: Presenting Analysis, Context, and Criticism on CommonlyRead MoreHaving Our Say Essay9582 Words   |  39 Pagesare trademarks used herein under license. The following sections, if they exist, are offprint from Beachams Encyclopedia of Popular Fiction: Social Concerns, Thematic Overview, Techniques, Literary Precedents, Key Questions, Related Titles, Adaptations, Related Web Sites. (c)1994-2005, by Walton Beacham. The following sections, if they exist, are offprint from Beachams Guide to Literature for Young Adults: About the Author, Overview, Setting, Literary Qualities, SocialRead MoreChildrens Literature13219 Words   |  53 Pagesarea of study. Its richness is reflected in the vast amount of theories that permeate and surround the term. From feminist studies to new historicism, literary theory places the child/text/context relationship on varying ideological and political axes. The reconceptualization of its history and the postmodern growth of radical alternative literary â€Å"histories† further complicate a retelling of the history of children’s literature. Consequently, it becomes not only a difficult but also a contentiousRead MoreJazz Albums as Art Essay4662 Words   |  19 Pagesalbums. According to the research of German art historian Martina Schmitz (whose 1987 book in German, ALBUM COVERS, is the definitive work on this subject),(2) before the mid-thirties, all re cord albums were plain, purely functional holders. What they used originally was either brown, gray, or tan paper, said Alex Steinweiss, who became Columbia Recordss first art director in 1940. They would stamp in gold the name of the record, and it would just lie in the window of the record store like a tombstone:

International Trade and B. Technology C. free essay sample

Both socialism and communism want to achieve a more equal distribution of income and wealth, but under communism the government takes more control over peoples lives. C. Socialism strives for an equal distribution of income but communism wants income to go primarily to those who own and operate private businesses. D. Communism believes that the government should always strive to balance its budget, while socialism accepts deficit spending as the price of achieving true equality. 16. A collapse in the national banking system of Lasia has resulted in a condition where prices are actually declining. This condition is known as: A. disinflation. B. deflation. C. contra inflation. D. overproduction. 17. One of the most significant problems faced by communist economies is: A. a great inequality in the distribution of wealth and income. B. severe inflationary pressures due to rapid economic growth. C. too little government regulation of economic activity. D. persistent shortages of basic goods such as food and clothing. 18. Thomas Malthus was pessimistic about the ability of societies to achieve high standards of living for most of their citizens because he believed that: A. the government had a natural tendency to run deficits that would eventually bankrupt the country. B. population growth would result in shortages of food and resources. C. owners of businesses would deliberately exploit their employees in an attempt to earn greater profits, and this would ultimately lead to a workers revolt. D. high tax rates would eventually undermine peoples incentives to work hard and create wealth. 19. Which of the following statements about the Federal Reserve System (the Fed) is most accurate? A. The Fed is an agency of the U. S. Treasury Department that has the responsibility of collecting tax receipts for the IRS. B. The Fed manages the U. S. money supply and interest rates. C. The Fed is the primary government agency involved in carrying out our nations fiscal policies. D. The Fed is the agency of the government that ensures the U. S. maintains enough gold reserves to pay any foreign debts that result from international trade. 20. The nation of Nadiria is in the midst of a terrible economic downturn. The nations GDP has been declining steadily for over five years and is now at its lowest level in over fifty years. Nadirias unemployment rate is at an all time high and the nations CPI is falling. Nadiria is experiencing a: A. period of stagflation. B. balance of trade deficit. C. depression. D. retraction. 21. Mortavia is a small nation that has followed protectionist policies for many years. Which of the following would be an example of a nontariff barrier established by Mortavia? A. Establishment of a tax levied on imported goods so that foreign products are more expensive than competing domestic goods. B. Establishment of a tax levied on imported goods designed primarily to raise money for the government. C. Establishment of restrictive quality standards requiring foreign firms to make expensive modifications in their products before they can be sold in Mortavia. D. Participation in the Uruguay Round of GATT negotiations. 22. The two basic types of tariffs are: A. goods and services. B. general and limited. C. comparative and absolute. D. revenue and protective. 23. __________ is an arrangement whereby someone with a good idea for a business sells the rights to use the business name and sell a product or service to others in a given territory in a specified manner. A. Franchising B. Contract manufacturing C. Import trading D. Export trading 4. The difference between money coming into a country (from exports) and money leaving the country (for imports) plus money flows from other factors such as tourism, foreign aid, and military expenditures is referred to as the: A. balance of payments. B. balance of trade. C. balance of money flows. D. exchange rate. 25. The largest importer in the glo bal market today is: A. Japan. B. Russia. C. Germany. D. the United States. 26. The emerging nation of Lancerland has enacted a tariff on imported petroleum in order to raise money for their new government. This trade restriction is an example of a(n) ________ tariff. A. revenue B. rotective C. percentage D. fixed 27. A Mexican firm has agreed to trade petroleum to an American importer in return for U. S. made computers. This arrangement is an example of: A. a letter of credit arrangement. B. a nontariff trade. C. arbitrage. D. barter. 28. The second wave of offshore outsourcing refers to: A. moving jobs to South America in addition to China. B. moving skilled service jobs such as those in accounting offshore. C. moving jobs to India in addition to China. D. moving manufacturing jobs offshore. 29. One advantage of free trade is: A. jobs are shifted to low-wage global markets. B. service jobs are moved overseas. C. advanced production operations are built in low-wage countries. D. innovation for new products occurs and keeps firms competitively challenged. 30. Casey is typical of many U. S. businesspeople. Casey feels the U. S. culture should be the model for the rest of the world, and that the American way of doing things is the best. Casey is guilty of: A. ethnic plagiarism. B. ethnocentricity. C. culture shock. D. counter culture. 31. After developing a code of ethics, it should be communicated to: A. everyone with whom the business has dealings. B. the Attorney General in jurisdictions where the business has operations. C. all levels of management. D. stockholders. 32. Management and employees at Boyer Enterprises are given time off each year to work in local charities. This firm clearly practices: A. corporate philanthropy. B. social irresponsibility. C. community mediation services. D. participative funding. 33. A friend offers to share with you a term paper she prepared for her Introduction to Business class last semester. When you tell her that it wouldnt be fair, she reminds you of your habit of reading an entire Harry Potter book at the local bookstore without paying for the book. Which ethics-based question would be the most helpful in evaluating these situations? A. Is it legal? B. Am I acting fairly? C. Who wins and who loses? D. Will I get caught? 34. Environmental quality is considered to be a public good. This means that: A. ultimately it is the public that pays for environmental clean up. B. businesses have a responsibility to minimize their environmental pollution. C. everyone enjoys a cleaner environment regardless of who pays for it. D. society places a higher value on private goods and services. 35. The first step when facing an ethical dilemma is to ask: A. Is it legal? B. Is it balanced? C. How will it make me feel about myself? D. Is it ethical? 36. When working with international firms, U. S. businesses: A. tend to ignore the ethics of their international partners. B. expect socially responsible behavior from their business partners. C. feel that their responsibility is limited to the United States. D. try to avoid judging our international business partners by U. S. standards. 37. Which of the following is the most important step to help improve Americas business ethics? A. middle management support B. training C. enforcement D. identification of the organizational ethics officer 8. The danger in writing new laws to correct behavior is that: A. people may begin to think that any behavior that is within the law is acceptable. B. people will see the difference between ethics and legality. C. legal behavior will reflect the totality of peoples proper relations with one another. D. there will not be enough jails to house those who break the law. 39. Critics of corporate social responsibility view spending money on CSR as: A. a necessary evil. B. stealing from investors. C. important for America. D. moving toward capitalism. 40. Obeying the law is ________ ethical behavior. A. essentially the same as B. the first step towards C. the opposite of D. unlikely to lead to 41. One of the newest items on the Cajun Cookin menu is a spicy Cajun-burger. Sondra was quite surprised to learn that how she made this burger was regulated by the: A. Federal Trade Commission. B. Food and Drug Administration. C. Federal Nutritional Association. D. State Board of Food Examiners. 42. One of the reasons you bought your mom a name-brand mixer for Mothers Day was that it came with a full warranty. This means that: A. the mixer is guaranteed to last for 10 years with normal use. B. the mixer will make wonderful baked goods as well as pasta. C. the mixer will be repaired or replaced at no charge if it is defective. D. only the motor is covered by the mixer manufacturer. 43. One result of deregulation in the airline industry has been: A. less intense competition. B. increase aviation related fatalities. C. higher prices for consumers. D. lower prices for consumers. 44. You agree to sell your car to your neighbor for $5,000. You and your neighbor have provided each other: A. duress. B. consideration. C. an endorsement. D. an expressed warranty. 45. Which of the following do school districts utilize to generate needed funding? A. sin tax B. sales tax C. property tax D. income tax 46. The remedy for a tort is: A. imprisonment. B. a breach of contract. C. bankruptcy. D. monetary compensation. 47. Which of the following protects the original work of authors and artists (i. e. , their books, photos, or cartoons)? A. a trademark B. a copyright C. the Uniform Commercial Code D. a patent 48. The Griggs v. Duke Power Company (1971) case established a precedent on the need to use job-related tests in employment practices. Similar court cases have been decided based on this decision. This is an example of: A. common law. B. statutory law. C. administrative law.

Sunday, April 19, 2020

There Are Many Different Life Forms On Earth. New Species And Differen

There are many different life forms on earth. New species and different sub species are evolving every second as others die out. In order to explain the origin of life, people have used the bible story of creation, while others came up with their own theories. Out of all those theories, the theory of evolution by Charles Darwin seems to have the most supporting evidence to back it up. Evidence on earth suggests that single cell creatures are the ancestors of all life. These organisms slowly evolved into air breathing creatures when oxygen was created. Slowly, the as land dried up, these creatures moved away from the water and on to land where they evolved legs and a strong back bone. The theory of evolution by Charles Darwin explained how life evolved from survival of the fittest, it provides a logical explanation of the evolutionary process these creatures took, and how human came to be. Evidence on earth shows that single cell organisms eventually evolved to multicelled organisms and complex beings. If you look at old fossil records, the oldest fossils are all single cell creatures. These simple creatures do not require oxygen to live. It took in energy from the sun and other energy sources. Slowly as oxygen begins to appear on Earth, some of the organisms began to evolve lungs to breathe oxygen because When free oxygen entered the picture. Breathing is more efficient than drawing energy from the fermentation process that earlier microscope life used. (Science: Biology Pg. 36) Other organisms that did not evolve lungs either died out or went down to deeper depths of the ocean; Oxygen was first consider a pollutant by microscopic creatures. (Evolution Pg. 36) Because of the free Oxygen those who did not change were killed. The evolution through natural selection and survival of the fittest shows that Darwin theory is correct. Due to the lack of oxygen within the ocean , fish slowly evolved to the condition of land. Due to the sudden increase in the population of fish and other marine organism. Due to the sudden increase in the population of marines life, the oxygen level within the ocean decreased. Scientist believe that the lung fish evolved because of a lack of oxygen in the sea. The lung fish learned to rise from the sea and breathe in the oxygen necessary for survival. (Evolution evidence Pg. 38) As more land emerges and dry, rain collected and formed fresh water lakes and rivers. Some of the fish became stranded by the receding ocean. Many fish that became stranded died because they were not able to adapt to the new environment; those fish needed salt water to lived died. But some adapted to their environment. (Evolution Pg. 38) The lobe fin fish was another evolutionary path fish toke to avoid the lack of oxygen in the sea. Fossil record shows that lobe finned lung fish took another evolutionary pat h. (Microsoft Encarta Pg. 2) The lobe fin fish was almost capable of leaving the water. It only needed more muscles in the joints to be able to lift it self up, because of this many people believe that the lobe fin fish was the ancestor of early reptilian creatures. The different evolutionary paths the fish have taken shows that life changes in responses to different environmental conditions. Fossil records in South America, reveals that there are many sub species of the human race. The fossil of Neanderthal man was discover shortly after Darwin published his book on evolution.. The Neanderthal man was consider to be an ancestor of mankind because of the size of the skull which suggested that it had a small brain. The sloping forehead is also an indication that it is a ancestor of the human race. Not long after the discovery of the Neanderthal was found in the 1890's. This fossil was called the Java Man, the Java Man was not consider the missing link between humans and apes. (Evolution Pg. 102) The Java Man was too alike a normal human to be the missing link. The missing link is a fossil of a creature that is in a transitional state between it original form and it new form.(I.e. A dinosaurs with

Sunday, March 15, 2020

Midsummer Nights Dream essays

Midsummer Nights Dream essays A Midsummer Nights Dream: The Movie The director of A Midsummer Nights Dream, Michael Hoffman, partially modernized the drama by changing it from 16th century in Greece to the turn of the 19th Century in Italy. The opening credits explain that it was a time when the bustle was on the wane and the bicycle was on the rise. These were considered harmonizing trends. These modernizations werent too modern, and his changes to the original story were minor and almost unnoticeable. But the film turned out a success with some effective touches here and there. The bicycle allowed for the characters to pedal after each other rather than chasing each other around on foot. It was a successful change that added a touch of humour and a little more interest to the story. At the beginning of the film we are servants preparing for Theseus wedding day. This instantly shows that Theseus has power and is high in society. A while after we are introduced to Helena. We see her walking down a road sulking and she comments on showers of ode did meet... As she is saying this its pouring rain. I thought that this was an interesting effect. It was definitely something that wasnt visualized in the book. We are then introduced to the mechanicals. The director of this film arranged for the camera to pass by Bottom the first time as a donkey walked in front of his face. Later in the film, Puck changes Bottoms head into that of an ass. Also, in the book, we were not introduced to Bottoms wife. In the film, she was added to give us some background about Bottom. She asks a person in the market Where is that worthless dreamer? This tells us right away that Bottoms head is always up in the clouds and he is a foolish thinker. Also, Bottoms wife is used to make the audience feel sympathetic towards him. Later on, after the gathering about the play, when he ...

Thursday, February 27, 2020

Develop Your Conflict Resolution Skills Essay Example | Topics and Well Written Essays - 750 words

Develop Your Conflict Resolution Skills - Essay Example Injustice: this is the perceived mistreatment by others or the world at large. This attitude leads to the individual to identify something as unfair and thereby engage in retaliatory acts. Vulnerability: it revolves around a person’s perception that he/she is the subject to internal/external dangers where control lacks to supply safety. Distrust: it focuses on presumed hostility and malign intent of others. This is an expectation that others will hurt, cheat, abuse, humiliate or take advantage. Helplessness: this is a conviction that the carefully planned and executed plans/actions will fail to produce desired outcomes. This leads to a person perceiving that he/she cannot accomplish anything. The conflict is solved when the inconsistency between actions and wishes of parties are resolved. Returning to the Sally and Dave conundrum, as the managers duty at the reservations call center is to ensure this conflict is resolved. Therefore, the managers first action after analyzing the conflict and putting all parties into consideration is to handle the issue separately, and find a solution after listening to each party’s complain. First, it is hard to tell who is telling the truth since each proposal is a complete opposite of the other party’s proposal. So, after meeting with Dave, the manager should ask him to be punctual with the time he departs from the center. He cannot stay at the center past 7:30. This would mean that he has to complete his job by seven o’clock thus giving him some time to tidy up the cubicle and turn off his computer. He should keep his stuff and personal effects to a minimized and limited to one side of the work station. Failure to honor this, he should be stripped of these permissions. He should keep his personal belongings on one side of the drawer and not leave them scattered in every corner of the drawer. Above all, responsibility and respect towards Sally would be demanded from Dave. Sally

Tuesday, February 11, 2020

Protein Quantitation Lab Report Example | Topics and Well Written Essays - 1250 words

Protein Quantitation - Lab Report Example The following is a report on an experiment conducted to determine protein concentration of unknown samples using this method. Bradford Protein Concentration Assay Accurate protein quantitation is paramount to all experiments that are related to proteins in a lot of research topics in molecular biology, developmental biology, cell biology, neuroscience, and biochemistry. Different techniques have been developed to quantitate proteins in the last century, both for the total protein content and a single protein. Total protein content quantitation methods include Bradford assays. Bradford assay, which was initially described by Dr. Marion Bradford in 1976, is one of the commonly used methods to determine protein concentration. This method relies on formation of a complex between proteins in solution and the Coomassie brilliant blue G-250 dye. This dye exists in four different ionic forms. The more anionic blue form binds to proteins and has an absorbance at 590 nm. Protein concentrations can be known by determining the amount of dye in the blue ionic form, and by measuring the absorbance of the solution at 595 nm using a spectrophotometer (Becker, Caldwell & Zachgo, 1996). This dye binds mostly to arginine, tryptophan, tyrosine, histidine, and phenylalanine residues of the protein Materials and Reagents Protein standard: 1mg/mL Albumin Bradford reagent Distilled water Test samples A, B and C (Unknown protein) One 96-well plate Procedure First, the albumin standard solutions were prepared as follows: Concentration Albumin Distilled Water 0% 0ul 100ul 25% 25ul 75ul 50% 50ul 50ul 75% 75ul 25ul 100% 100ul 0ul Then the Bradford reagents were diluted with 300 ul (Bradford): 1500ul (Distill Water) (1:5 ratio). In the first trial, 180ul of the diluted Bradford reagents was added into the 96 well plate. Then 0%, 25%, 50%, 75%, 100% and Sample A, B, C, each 5ul was added to each well hole which contained 180ul of diluted Bradford reagent. These were tested with spectrophotom eter and the results recorded. The experiment was repeated again the same way. In addition, Sample C was diluted with 3 different ratios as follows: 1:9 (Sample C: Distill Water) 1:99 (Sample C: Distill Water) 5:95 (Sample C: Distill Water) The results were recorded after the solutions were tested with a spectrophotometer. A standard curve of absorbance versus concentration protein was drawn. Results The results were recorded as follows: First Trial Results: 0% 25% 50% 75% 100% Sample A Sample B Sample C 0.092 0.145 0.161 0.169 0.162 0.095 0.161 0.763 Second Trial Results: 0% 25% 50% 75% 100% Sample A Sample B Sample C Sample C (1:9) Sample C (1:99) Sample C (5:95) 0.113 0.188 0.218 0.217 0.210 0.116 0.200 0.670 0.244 0.118 0.152 The responses of the standards were used to plot a standard curve. Absorbance values of unknown samples were then interpolated onto the plot for the standard curve to determine their concentrations as shown in the graph below. Discussion From the graph abov e, the optimum measurement wavelength for this assay is on sample C. Thus sample C has the highest protein concentration. Sample A has the same absorbance as 0% albumin and Sample B has the same absorbance as 50% albumin. It can therefore be concluded that sample A and Sample B have 0% and 50% protein concentra